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In vitro evolution of horse heart myoglobin to increase peroxidase activity

Random mutagenesis and screening for enzymatic activity has been used to engineer horse heart myoglobin to enhance its intrinsic peroxidase activity. A chemically synthesized gene encoding horse heart myoglobin was subjected to successive cycles of PCR random mutagenesis. The mutated myoglobin gene...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Wan, Lianglu, Twitchett, Mark B., Eltis, Lindsay D., Mauk, A. Grant, Smith, Michael
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1998
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC23613/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9788999/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.22.12825
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