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Global analysis of three-state protein unfolding data

A new method for analyzing three-state protein unfolding equilibria is described that overcomes the difficulties created by direct effects of denaturants on circular dichroism (CD) and fluorescence spectra of the intermediate state. The procedure begins with a singular value analysis of the data mat...

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Detalhes bibliográficos
Main Authors: Harder, Mark E., Deinzer, Max L., Leid, Mark E., Schimerlik, Michael I.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279808/
https://ncbi.nlm.nih.gov/pubmed/15273314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.03229504
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