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Hydrogen/deuterium exchange and mass spectrometric analysis of a protein containing multiple disulfide bonds: Solution structure of recombinant macrophage colony stimulating factor-beta (rhM-CSFβ)

Studies with the homodimeric recombinant human macrophage colony-stimulating factor beta (rhM-CSFβ), show for the first time that a large number (9) of disulfide linkages can be reduced after amide hydrogen/deuterium (H/D) exchange, and the protein digested and analyzed successfully for the isotopic...

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Detalhes bibliográficos
Main Authors: Yan, Xuguang, Zhang, Heidi, Watson, Jeffrey, Schimerlik, Michael I., Deinzer, Max L.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373587/
https://ncbi.nlm.nih.gov/pubmed/12192067
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