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Simultaneous Reduction and Digestion of Proteins with Disulfide Bonds for Hydrogen/Deuterium Exchange Monitored by Mass Spectrometry

Proteolyzed peptides provide the basis for mass-analyzed hydrogen/deuterium exchange (HDX) for mapping solvent access to various segments of solution-phase proteins. Aspergillus saitoi protease type XIII and porcine pepsin can generate peptides of overlapping sequences and high sequence coverage. Ho...

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Détails bibliographiques
Auteurs principaux: Zhang, Hui-Min, McLoughlin, Shaun M., Frausto, Stephen D., Tang, Hengli, Emmett, Mark R., Marshall, Alan G.
Format: Artigo
Langue:Inglês
Publié: 2010
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2825376/
https://ncbi.nlm.nih.gov/pubmed/20099838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac902550n
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