טוען...
Simultaneous optimization of enzyme activity and quaternary structure by directed evolution
Natural evolution has produced efficient enzymes of enormous structural diversity. We imitated this natural process in the laboratory to augment the efficiency of an engineered chorismate mutase with low activity and an unusual hexameric topology. By applying two rounds of DNA shuffling and genetic...
שמור ב:
| Main Authors: | , , , , |
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| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Cold Spring Harbor Laboratory Press
2005
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2279322/ https://ncbi.nlm.nih.gov/pubmed/15987889 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051431605 |
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