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Searching sequence space for protein catalysts

Genetic selection was used to explore the probability of finding enzymes in protein sequence space. Large degenerate libraries were prepared by replacing all secondary structure units in a dimeric, helical bundle chorismate mutase with simple binary-patterned modules based on a limited set of four p...

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Detalhes bibliográficos
Main Authors: Taylor, Sean V., Walter, Kai U., Kast, Peter, Hilvert, Donald
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC58511/
https://ncbi.nlm.nih.gov/pubmed/11535813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.191159298
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