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Directed evolution of a thermostable esterase

We have used in vitro evolution to probe the relationship between stability and activity in a mesophilic esterase. Previous studies of these properties in homologous enzymes evolved for function at different temperatures have suggested that stability at high temperatures is incompatible with high ca...

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Detalhes bibliográficos
Main Authors: Giver, Lori, Gershenson, Anne, Freskgard, Per-Ola, Arnold, Frances H.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC23604/
https://ncbi.nlm.nih.gov/pubmed/9788996
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