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Simultaneous optimization of enzyme activity and quaternary structure by directed evolution
Natural evolution has produced efficient enzymes of enormous structural diversity. We imitated this natural process in the laboratory to augment the efficiency of an engineered chorismate mutase with low activity and an unusual hexameric topology. By applying two rounds of DNA shuffling and genetic...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2279322/ https://ncbi.nlm.nih.gov/pubmed/15987889 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.051431605 |
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