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Hydrogen-Deuterium Exchange Mass Spectrometry for Investigation of Backbone Dynamics of Oxidized and Reduced Cytochrome P450(cam)

Backbone dynamics of the camphor monoxygenase cytochrome P450(cam) (CYP101) as a function of oxidation/ligation state of the heme iron were investigated via hydrogen/deuterium exchange (H/D exchange) as monitored by mass spectrometry. Main chain amide NH hydrogens can exchange readily with solvent a...

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Main Authors: Hamuro, Yoshitomo, Molnar, Kathleen S., Coales, Stephen J., OuYang, Bo, Simorellis, Alana, Pochapsky, Thomas C.
Format: Artigo
Jezik:Inglês
Izdano: 2007
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279175/
https://ncbi.nlm.nih.gov/pubmed/18023482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jinorgbio.2007.10.001
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