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Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates
We describe a method for rapid identification of protein kinase substrates. Cdk1 was engineered to accept an ATP analog that allows it to uniquely label its substrates with a bio-orthogonal phosphate analog tag. A highly specific, covalent capture-and-release methodology was developed for rapid puri...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
2008
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2234163/ https://ncbi.nlm.nih.gov/pubmed/18234856 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0708966105 |
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