Chemical Genetic Approach for Kinase-Substrate Mapping by Covalent Capture of Thiophosphopeptides and Analysis by Mass Spectrometry

Mapping kinase-substrate interactions demands robust methods to rapidly and unequivocally identify substrates from complex protein mixtures. Towards this goal we present a method in which a kinase, engineered to utilize synthetic ATPγS analogs, specifically thiophosphorylates its substrates in a com...

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Hauptverfasser: Hertz, Nicholas T., Wang, Beatrice T., Allen, Jasmina J., Zhang, Chao, Dar, Arvin C., Burlingame, Alma L., Shokat, Kevan M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3894912/
https://ncbi.nlm.nih.gov/pubmed/23836541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470559277.ch090201
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