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Combining site-specific mutagenesis and seeding as a strategy to crystallize ‘difficult’ proteins: the case of Staphylococcus aureus thioredoxin

The P31T mutant of Staphylococcus aureus thioredoxin crystallizes spontaneously in space group P2(1)2(1)2(1), with unit-cell parameters a = 41.7, b = 49.5, c = 55.6 Å. The crystals diffract to 2.2 Å resolution. Isomorphous crystals of wild-type thioredoxin as well as of other point mutants only grow...

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Bibliografski detalji
Glavni autori: Roos, Goedele, Brosens, Elke, Wahni, Khadija, Desmyter, Aline, Spinelli, Silvia, Wyns, Lode, Messens, Joris, Loris, Remy
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2006
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Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225371/
https://ncbi.nlm.nih.gov/pubmed/17142910
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106047075
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