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A continuous hyperchromicity assay to characterize the kinetics and thermodynamics of DNA lesion recognition and base excision
We report a continuous hyperchromicity assay (CHA) for monitoring and characterizing enzyme activities associated with DNA processing. We use this assay to determine kinetic and thermodynamic parameters for a repair enzyme that targets nucleic acid substrates containing a specific base lesion. This...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2224234/ https://ncbi.nlm.nih.gov/pubmed/18172202 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0710363105 |
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