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The thermodynamics of template-directed DNA synthesis: Base insertion and extension enthalpies
We used stopped-flow calorimetry to measure the overall enthalpy change associated with template-directed nucleotide insertion and DNA extension. Specifically, we used families of hairpin self-priming templates in conjunction with an exonuclease-free DNA polymerase to study primer extension by one o...
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| Hlavní autoři: | , , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
2003
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC299778/ https://ncbi.nlm.nih.gov/pubmed/14623953 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2336142100 |
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