The thermodynamics of template-directed DNA synthesis: Base insertion and extension enthalpies

We used stopped-flow calorimetry to measure the overall enthalpy change associated with template-directed nucleotide insertion and DNA extension. Specifically, we used families of hairpin self-priming templates in conjunction with an exonuclease-free DNA polymerase to study primer extension by one o...

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Detalhes bibliográficos
Main Authors: Minetti, Conceição A. S. A., Remeta, David P., Miller, Holly, Gelfand, Craig A., Plum, G. Eric, Grollman, Arthur P., Breslauer, Kenneth J.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2003
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC299778/
https://ncbi.nlm.nih.gov/pubmed/14623953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2336142100
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