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Bypassing the periplasm: Reconstitution of the AcrAB multidrug efflux pump of Escherichia coli

AcrAB is a constitutively expressed, major multidrug efflux system of Escherichia coli. We have purified the cytoplasmic membrane component, AcrB, to near homogeneity, and reconstituted the protein into proteoliposomes. In the presence of ΔpH (outside acid), the protein catalyzed the extrusion of fl...

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發表在:Proc Natl Acad Sci U S A
Principais autores: Zgurskaya, Helen I., Nikaido, Hiroshi
格式: Artigo
語言:Inglês
出版: National Academy of Sciences 1999
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在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC22048/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10377390/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.13.7190
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