Bypassing the periplasm: Reconstitution of the AcrAB multidrug efflux pump of Escherichia coli
AcrAB is a constitutively expressed, major multidrug efflux system of Escherichia coli. We have purified the cytoplasmic membrane component, AcrB, to near homogeneity, and reconstituted the protein into proteoliposomes. In the presence of ΔpH (outside acid), the protein catalyzed the extrusion of fl...
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| 發表在: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
National Academy of Sciences
1999
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC22048/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10377390/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.13.7190 |
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