Bypassing the periplasm: Reconstitution of the AcrAB multidrug efflux pump of Escherichia coli
AcrAB is a constitutively expressed, major multidrug efflux system of Escherichia coli. We have purified the cytoplasmic membrane component, AcrB, to near homogeneity, and reconstituted the protein into proteoliposomes. In the presence of ΔpH (outside acid), the protein catalyzed the extrusion of fl...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1999
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC22048/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10377390/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.13.7190 |
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