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Bypassing the periplasm: Reconstitution of the AcrAB multidrug efflux pump of Escherichia coli

AcrAB is a constitutively expressed, major multidrug efflux system of Escherichia coli. We have purified the cytoplasmic membrane component, AcrB, to near homogeneity, and reconstituted the protein into proteoliposomes. In the presence of ΔpH (outside acid), the protein catalyzed the extrusion of fl...

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Autors principals: Zgurskaya, Helen I., Nikaido, Hiroshi
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC22048/
https://ncbi.nlm.nih.gov/pubmed/10377390
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