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Highly specific zinc finger proteins obtained by directed domain shuffling and cell-based selection

Engineered Cys(2)His(2) zinc finger proteins (ZFPs) can mediate regulation of endogenous gene expression in mammalian cells. Ideally, all zinc fingers in an engineered multifinger protein should be optimized concurrently because cooperative and context-dependent contacts can affect DNA recognition....

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Autors principals: Hurt, Jessica A., Thibodeau, Stacey A., Hirsh, Andrew S., Pabo, Carl O., Joung, J. Keith
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC218748/
https://ncbi.nlm.nih.gov/pubmed/14527993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2135381100
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