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A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C

Signals transduced by kinases depend on the extent and duration of substrate phosphorylation. We generated genetically encoded fluorescent reporters for PKC activity that reversibly respond to stimuli activating PKC. Specifically, phosphorylation of the reporter expressed in mammalian cells causes c...

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Detalles Bibliográficos
Main Authors: Violin, Jonathan D., Zhang, Jin, Tsien, Roger Y., Newton, Alexandra C.
Formato: Artigo
Idioma:Inglês
Publicado: The Rockefeller University Press 2003
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2172956/
https://ncbi.nlm.nih.gov/pubmed/12782683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.200302125
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