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Transcription-coupled Hypernegative Supercoiling of Plasmid DNA by T7 RNA Polymerase in Escherichia coli Topoisomerase I Deficient Strains
Transcription by RNA polymerase can stimulate negative DNA supercoiling in Escherichia coli topA strains. This phenomenon has been explained by a “twin-supercoiled-domain” model of transcription in which positive DNA supercoils are generated in front of a translocating RNA polymerase and negative su...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2151476/ https://ncbi.nlm.nih.gov/pubmed/17980389 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2007.10.011 |
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