Method for localization of cloned DNA fragments on the Escherichia coli chromosome.

In exponentially growing cultures of Escherichia coli strains carrying the dnaC28 mutation, DNA replication can be synchronized by temperature changes (R. L. Rodriguez, M. S. Dalbey, and C. I. Davern, J. Mol. Biol. 74:599-604, 1973). We used this synchronization procedure and DNA-DNA hybridization t...

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Bibliographic Details
Main Authors: Fayet, O, Prere, M F
Format: Artigo
Language:Inglês
Published: 1987
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC214021/
https://ncbi.nlm.nih.gov/pubmed/3316190
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