Method for localization of cloned DNA fragments on the Escherichia coli chromosome.
In exponentially growing cultures of Escherichia coli strains carrying the dnaC28 mutation, DNA replication can be synchronized by temperature changes (R. L. Rodriguez, M. S. Dalbey, and C. I. Davern, J. Mol. Biol. 74:599-604, 1973). We used this synchronization procedure and DNA-DNA hybridization t...
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| 出版年: | J Bacteriol |
|---|---|
| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology (ASM)
1987
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214021/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3316190/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5641-5647.1987 |
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