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Method for localization of cloned DNA fragments on the Escherichia coli chromosome.

In exponentially growing cultures of Escherichia coli strains carrying the dnaC28 mutation, DNA replication can be synchronized by temperature changes (R. L. Rodriguez, M. S. Dalbey, and C. I. Davern, J. Mol. Biol. 74:599-604, 1973). We used this synchronization procedure and DNA-DNA hybridization t...

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Bibliografische gegevens
Gepubliceerd in:J Bacteriol
Hoofdauteurs: Fayet, O, Prere, M F
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology (ASM) 1987
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214021/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3316190/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5641-5647.1987
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