Method for localization of cloned DNA fragments on the Escherichia coli chromosome.

In exponentially growing cultures of Escherichia coli strains carrying the dnaC28 mutation, DNA replication can be synchronized by temperature changes (R. L. Rodriguez, M. S. Dalbey, and C. I. Davern, J. Mol. Biol. 74:599-604, 1973). We used this synchronization procedure and DNA-DNA hybridization t...

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Bibliographic Details

Published in:	J Bacteriol
Main Authors:	Fayet, O, Prere, M F
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 1987
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214021/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3316190/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5641-5647.1987
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