Method for localization of cloned DNA fragments on the Escherichia coli chromosome.

In exponentially growing cultures of Escherichia coli strains carrying the dnaC28 mutation, DNA replication can be synchronized by temperature changes (R. L. Rodriguez, M. S. Dalbey, and C. I. Davern, J. Mol. Biol. 74:599-604, 1973). We used this synchronization procedure and DNA-DNA hybridization t...

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Publié dans:	J Bacteriol
Auteurs principaux:	Fayet, O, Prere, M F
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1987
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214021/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3316190/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5641-5647.1987
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