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A method for the construction of equalized directional cDNA libraries from hydrolyzed total RNA

BACKGROUND: The transcribed sequences of a cell, the transcriptome, represent the trans-acting fraction of the genetic information, yet eukaryotic cDNA libraries are typically made from only the poly-adenylated fraction. The non-coding or translated but non-polyadenylated RNAs are therefore not repr...

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Autors principals: Davis, Claytus, Barvish, Zeev, Gitelman, Inna
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2007
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2134933/
https://ncbi.nlm.nih.gov/pubmed/17925018
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-8-363
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