Generation of cDNA expression libraries enriched for in-frame sequences
Bacterial cDNA expression libraries are made to reproduce protein sequences present in the mRNA source tissue. However, there is no control over which frame of the cDNA is translated, because translation of the cDNA must be initiated on vector sequence. In a library of nondirectionally cloned cDNAs,...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC20052/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9122159/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.94.6.2128 |
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