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Generation of cDNA expression libraries enriched for in-frame sequences

Bacterial cDNA expression libraries are made to reproduce protein sequences present in the mRNA source tissue. However, there is no control over which frame of the cDNA is translated, because translation of the cDNA must be initiated on vector sequence. In a library of nondirectionally cloned cDNAs,...

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Detalhes bibliográficos
Main Authors: Davis, Claytus A., Benzer, Seymour
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences of the USA 1997
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC20052/
https://ncbi.nlm.nih.gov/pubmed/9122159
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