Directional cDNA library construction assisted by the in vitro recombination reaction

We report here a new directional cDNA library construction method using an in vitro site-specific recombination reaction, based on the integrase–excisionase system of bacteriophage λ. Preliminary experiments revealed that in vitro recombinational cloning (RC) provided important advantages over conve...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Ohara, Osamu, Temple, Gary
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2001
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29629/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11160942/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.4.e22
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