Isolation of anti-angiogenesis antibodies from a large combinatorial repertoire by colony filter screening
We describe here a method, based on iterative colony filter screening, for the rapid isolation of binding specificities from a large synthetic repertoire of human antibody fragments in single-chain Fv configuration. Escherichia coli cells, expressing the library of antibody fragments, are grown on a...
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| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2001
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29740/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11222778/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.5.e27 |
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