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Isolation of anti-angiogenesis antibodies from a large combinatorial repertoire by colony filter screening

We describe here a method, based on iterative colony filter screening, for the rapid isolation of binding specificities from a large synthetic repertoire of human antibody fragments in single-chain Fv configuration. Escherichia coli cells, expressing the library of antibody fragments, are grown on a...

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Bibliografske podrobnosti
Main Authors: Giovannoni, Leonardo, Viti, Francesca, Zardi, Luciano, Neri, Dario
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2001
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC29740/
https://ncbi.nlm.nih.gov/pubmed/11222778
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