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Construction of sized eukaryotic cDNA libraries using low input of total environmental metatranscriptomic RNA
BACKGROUND: Construction of high quality cDNA libraries from the usually low amounts of eukaryotic mRNA extracted from environmental samples is essential in functional metatranscriptomics for the selection of functional, full-length genes encoding proteins of interest. Many of the inserts in librari...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4170940/ https://ncbi.nlm.nih.gov/pubmed/25183040 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-14-80 |
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