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A method for the construction of equalized directional cDNA libraries from hydrolyzed total RNA
BACKGROUND: The transcribed sequences of a cell, the transcriptome, represent the trans-acting fraction of the genetic information, yet eukaryotic cDNA libraries are typically made from only the poly-adenylated fraction. The non-coding or translated but non-polyadenylated RNAs are therefore not repr...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2007
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2134933/ https://ncbi.nlm.nih.gov/pubmed/17925018 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-8-363 |
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