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Single-Pair FRET Microscopy Reveals Mononucleosome Dynamics
We applied spFRET microscopy for direct observation of intranucleosomal DNA dynamics. Mononucleosomes, reconstituted with DNA containing a FRET pair at the dyad axis and exit of the nucleosome core particle, were immobilized through a 30 bp DNA tether on a polyethyleneglycol functionalized slide and...
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| Main Authors: | , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Springer US
2007
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2064943/ https://ncbi.nlm.nih.gov/pubmed/17609864 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10895-007-0218-9 |
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