Single-Pair FRET Microscopy Reveals Mononucleosome Dynamics

We applied spFRET microscopy for direct observation of intranucleosomal DNA dynamics. Mononucleosomes, reconstituted with DNA containing a FRET pair at the dyad axis and exit of the nucleosome core particle, were immobilized through a 30 bp DNA tether on a polyethyleneglycol functionalized slide and...

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Main Authors: Koopmans, W. J. A., Brehm, A., Logie, C., Schmidt, T., van Noort, J.
Format: Artigo
Jezik:Inglês
Izdano: Springer US 2007
Teme:
Original Paper
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2064943/
https://ncbi.nlm.nih.gov/pubmed/17609864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10895-007-0218-9
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