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Intramolecular Three-Colour Single Pair FRET of Intrinsically Disordered Proteins with Increased Dynamic Range

Single molecule observation of fluorescence resonance energy transfer can be used to provide insights into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions wit...

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Detalhes bibliográficos
Main Authors: Milles, Sigrid, Koehler, Christine, Gambin, Yann, Deniz, Ashok A, Lemke, Edward A
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3441834/
https://ncbi.nlm.nih.gov/pubmed/22739670
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c2mb25135c
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