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Enzyme kinetic modelling as a tool to analyse the behaviour of cytochrome P450 catalysed reactions: application to amitriptyline N-demethylation

1To determine kinetic parameters (V(max), K(m)) for cytochrome P450 (CYP) mediated metabolic pathways, nonlinear least squares regression is commonly used to fit a model equation (e.g., Michaelis Menten [MM]) to sets of data points (reaction velocity vs substrate concentration). This method can also...

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Detalhes bibliográficos
Main Authors: SCHMIDER, JÜRGEN, GREENBLATT, DAVID J., HARMATZ, JEROLD S., SHADER, RICHARD I.
Formato: Artigo
Idioma:Inglês
Publicado em: 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2042612/
https://ncbi.nlm.nih.gov/pubmed/8799527
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1046/j.1365-2125.1996.35717.x
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