Directed evolution of an aspartate aminotransferase with new substrate specificities
The substrate specificity of aspartate aminotransferase was successfully modified by directed molecular evolution using a combination of DNA shuffling and selection in an auxotrophic Escherichia coli strain. After five rounds of selection, one of the evolved mutants showed a 10(5)-fold increase in t...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
|---|---|
| Principais autores: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1998
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC20408/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9576913/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.10.5511 |
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