Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity
Single-chain antibody mutants have been evolved in vitro with antigen-binding equilibrium dissociation constant K(d) = 48 fM and slower dissociation kinetics (half-time > 5 days) than those for the streptavidin–biotin complex. These mutants possess the highest monovalent ligand-binding affinity yet...
Guardado en:
| Publicado en: | Proc Natl Acad Sci U S A |
|---|---|
| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
2000
|
| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC27086/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10984501/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.170297297 |
| Etiquetas: |
Sin Etiquetas, Sea el primero en etiquetar este registro!
|