Directed evolution of an aspartate aminotransferase with new substrate specificities

The substrate specificity of aspartate aminotransferase was successfully modified by directed molecular evolution using a combination of DNA shuffling and selection in an auxotrophic Escherichia coli strain. After five rounds of selection, one of the evolved mutants showed a 10(5)-fold increase in t...

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Podrobná bibliografie

Vydáno v:	Proc Natl Acad Sci U S A
Hlavní autoři:	Yano, Takato, Oue, Shinya, Kagamiyama, Hiroyuki
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	National Academy of Sciences 1998
Témata:	Biological Sciences
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC20408/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9576913/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.10.5511
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