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Site-saturation Mutagenesis is more Efficient than DNA Shuffling for the Directed Evolution of β-Fucosidase from β-Galactosidase

Protein engineers use a variety of mutagenic strategies to adapt enzymes to novel substrates. Directed evolution techniques (random mutagenesis and high-throughput screening) offer a systematic approach to the management of protein complexity. This sub-discipline was galvanized by the invention of D...

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Bibliografski detalji
Glavni autori: Parikh, Monal R., Matsumura, Ichiro
Format: Artigo
Jezik:Inglês
Izdano: 2005
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2014722/
https://ncbi.nlm.nih.gov/pubmed/16095606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2005.07.020
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