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Site-directed mutagenesis of a family 42 β-galactosidase from an antarctic bacterium

Site directed mutagenesis was used to modify the active site of a cold active beta-galactosidase taken from an Antarctic psychrotolerant Planococcus Bacterial isolate. The goal was to modify the active site such that there would be an increase in activity on certain substrates which showed little to...

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Detalhes bibliográficos
Main Authors: Shumway, Matthew V, Sheridan, Peter P
Formato: Artigo
Idioma:Inglês
Publicado em: e-Century Publishing Corporation 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388732/
https://ncbi.nlm.nih.gov/pubmed/22773960
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