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Detection of changes in pairwise interactions during allosteric transitions: Coupling between local and global conformational changes in GroEL

A protein engineering approach for detecting and measuring local conformational changes that accompany allosteric transitions in proteins is described. Using this approach, we can identify interactions that are made or broken during allosteric transitions. The method is applied to probe for changes...

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Detalhes bibliográficos
Main Authors: Aharoni, Amir, Horovitz, Amnon
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences of the USA 1997
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC19979/
https://ncbi.nlm.nih.gov/pubmed/9050841
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