Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I
We present a system for random mutagenesis in Escherichia coli for the evolution of targeted genes. To increase error rates of DNA polymerase I (Pol I) replication, we introduced point mutations in three structural domains that govern Pol I fidelity. Expression of error-prone Pol I in vivo results i...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2003
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC187833/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12909725/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.1333928100 |
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