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Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I

We present a system for random mutagenesis in Escherichia coli for the evolution of targeted genes. To increase error rates of DNA polymerase I (Pol I) replication, we introduced point mutations in three structural domains that govern Pol I fidelity. Expression of error-prone Pol I in vivo results i...

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Bibliografiske detaljer
Main Authors: Camps, Manel, Naukkarinen, Jussi, Johnson, Ben P., Loeb, Lawrence A.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC187833/
https://ncbi.nlm.nih.gov/pubmed/12909725
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1333928100
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