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Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I
We present a system for random mutagenesis in Escherichia coli for the evolution of targeted genes. To increase error rates of DNA polymerase I (Pol I) replication, we introduced point mutations in three structural domains that govern Pol I fidelity. Expression of error-prone Pol I in vivo results i...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
2003
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC187833/ https://ncbi.nlm.nih.gov/pubmed/12909725 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1333928100 |
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