Nalaganje...
Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I
We present a system for random mutagenesis in Escherichia coli for the evolution of targeted genes. To increase error rates of DNA polymerase I (Pol I) replication, we introduced point mutations in three structural domains that govern Pol I fidelity. Expression of error-prone Pol I in vivo results i...
Shranjeno v:
| Main Authors: | , , , |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
2003
|
| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC187833/ https://ncbi.nlm.nih.gov/pubmed/12909725 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1333928100 |
| Oznake: |
Označite
Brez oznak, prvi označite!
|