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Acid–base catalysis in Leuconostoc mesenteroides sucrose phosphorylase probed by site-directed mutagenesis and detailed kinetic comparison of wild-type and Glu(237)→Gln mutant enzymes
The role of acid–base catalysis in the two-step enzymatic mechanism of α-retaining glucosyl transfer by Leuconostoc mesenteroides sucrose phosphorylase has been examined through site-directed replacement of the putative catalytic Glu(237) and detailed comparison of purified wild-type and Glu(237)→Gl...
Gorde:
| Egile Nagusiak: | , , |
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| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
Portland Press Ltd.
2007
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1876375/ https://ncbi.nlm.nih.gov/pubmed/17233628 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20070042 |
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