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Acid–base catalysis in Leuconostoc mesenteroides sucrose phosphorylase probed by site-directed mutagenesis and detailed kinetic comparison of wild-type and Glu(237)→Gln mutant enzymes
The role of acid–base catalysis in the two-step enzymatic mechanism of α-retaining glucosyl transfer by Leuconostoc mesenteroides sucrose phosphorylase has been examined through site-directed replacement of the putative catalytic Glu(237) and detailed comparison of purified wild-type and Glu(237)→Gl...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Portland Press Ltd.
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1876375/ https://ncbi.nlm.nih.gov/pubmed/17233628 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20070042 |
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