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An efficient recombination system for chromosome engineering in Escherichia coli

A recombination system has been developed for efficient chromosome engineering in Escherichia coli by using electroporated linear DNA. A defective λ prophage supplies functions that protect and recombine an electroporated linear DNA substrate in the bacterial cell. The use of recombination eliminate...

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Main Authors: Yu, Daiguan, Ellis, Hilary M., Lee, E-Chiang, Jenkins, Nancy A., Copeland, Neal G., Court, Donald L.
Formáid: Artigo
Teanga:Inglês
Foilsithe: The National Academy of Sciences 2000
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Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC18544/
https://ncbi.nlm.nih.gov/pubmed/10811905
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