An efficient recombination system for chromosome engineering in Escherichia coli

A recombination system has been developed for efficient chromosome engineering in Escherichia coli by using electroporated linear DNA. A defective λ prophage supplies functions that protect and recombine an electroporated linear DNA substrate in the bacterial cell. The use of recombination eliminate...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Yu, Daiguan, Ellis, Hilary M., Lee, E-Chiang, Jenkins, Nancy A., Copeland, Neal G., Court, Donald L.
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 2000
Sujets:	Biological Sciences
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC18544/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10811905/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.100127597
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