An efficient recombination system for chromosome engineering in Escherichia coli

A recombination system has been developed for efficient chromosome engineering in Escherichia coli by using electroporated linear DNA. A defective λ prophage supplies functions that protect and recombine an electroporated linear DNA substrate in the bacterial cell. The use of recombination eliminate...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Yu, Daiguan, Ellis, Hilary M., Lee, E-Chiang, Jenkins, Nancy A., Copeland, Neal G., Court, Donald L.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2000
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC18544/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10811905/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.100127597
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!