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Recombineering with overlapping single-stranded DNA oligonucleotides: Testing a recombination intermediate
A phage λ-based recombination system, Red, can be used for high-efficiency mutagenesis, repair, and engineering of chromosomal or episomal DNA in vivo in Escherichia coli. When long linear double-stranded DNA with short flanking homologies to their targets are used for the recombination, the λ Exo,...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC165854/ https://ncbi.nlm.nih.gov/pubmed/12771385 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1232375100 |
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