Recombineering with overlapping single-stranded DNA oligonucleotides: Testing a recombination intermediate

A phage λ-based recombination system, Red, can be used for high-efficiency mutagenesis, repair, and engineering of chromosomal or episomal DNA in vivo in Escherichia coli. When long linear double-stranded DNA with short flanking homologies to their targets are used for the recombination, the λ Exo,...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Yu, Daiguan, Sawitzke, James A., Ellis, Hilary, Court, Donald L.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2003
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC165854/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12771385/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.1232375100
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